Franz Arnold Ake

Franz Arnold Ake

Postdoctoral research scientist, Department of Pathology, Stanford University

Methanol fixation is the method of choice for droplet-based single-cell transcriptomics of neural cells

Published in Communications Biology, 2023

Abstract

This study provides a systematic comparison of fixation methods for droplet-based single-cell RNA sequencing of neural cells. We demonstrate that methanol fixation preserves transcriptional profiles more accurately than alternative approaches, establishing best practices for single-cell studies of neural tissue.

Key Highlights

  • Systematic evaluation of fixation methods for scRNA-seq
  • Methanol fixation preserves transcriptional integrity
  • Application to neural cell types
  • Protocol optimization for droplet-based platforms

Impact

This work has been cited 21 times and provides practical guidance for researchers performing single-cell transcriptomics on neural samples.

Citation

Citations: 21 (Google Scholar)

@article{gutierrez2023methanol,
  title={Methanol fixation is the method of choice for droplet-based single-cell transcriptomics of neural cells},
  author={Guti{\'e}rrez-Franco, A and Ake, Franz and Hassan, MN and Cayuela, NC and Mularoni, L and Plass, M},
  journal={Communications Biology},
  volume={6},
  number={1},
  pages={522},
  year={2023},
  publisher={Nature Publishing Group}
}

Recommended citation: Gutiérrez-Franco, A., Ake, F., Hassan, M.N., Cayuela, N.C., Mularoni, L., Plass, M. (2023). Methanol fixation is the method of choice for droplet-based single-cell transcriptomics of neural cells. Communications Biology, 6(1), 522.
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